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人原代肝臟細(xì)胞培養(yǎng)基
人原代肝臟細(xì)胞（Human primary hepatocytes, hphep) 是肝臟疾病毒理學(xué)研究和相關(guān)藥物研發(fā)的有力工具。對(duì)肝臟疾病模型研究和肝毒性檢測(cè)而言，一個(gè)穩(wěn)健的hphep培養(yǎng)體系至關(guān)重要。然而，當(dāng)前可用的培養(yǎng)體系培養(yǎng)的肝臟細(xì)胞在一周內(nèi)就喪失了功能性和活性，嚴(yán)重限制了原代肝臟細(xì)胞的應(yīng)用。而且，這些培養(yǎng)基維持培養(yǎng)中還需要每天換液。
Cellartis Power Primary HEP Medium是專門制定的、用于長(zhǎng)期維持培養(yǎng)的人原代肝臟細(xì)胞培養(yǎng)基。凍存復(fù)蘇后接種的hphep，在方便操作的2D培養(yǎng)中，可以存活并維持功能特性4周。此外，這款培養(yǎng)基進(jìn)一步簡(jiǎn)化了肝細(xì)胞培養(yǎng)，提供一個(gè)周末免換液的培養(yǎng)日程。
Cellartis Power Primary HEP Medium
Human primary hepatocytes (hphep) are an invaluable tool for academic and pharmaceutical labs studying liver disease pathology or as part of the drug development pipeline. To assess disease models and hepatocyte toxicity, robust and predictive hphep cell cultures are required. However, currently available culture media severely limits the utility of primary hepatocytes because hepatocytes lose their function and viability within a week. Additionally, daily feedings are required to maintain the hphep cultures.
產(chǎn)品特點(diǎn)
☆延長(zhǎng)培養(yǎng)時(shí)間—原代肝臟細(xì)胞可以存活并維持功能特性長(zhǎng)達(dá)4周，經(jīng)過CYP 活性、 albumin分泌和CYP誘導(dǎo)能力檢測(cè)驗(yàn)證。
☆形式靈活—冷凍運(yùn)輸，可以被分裝和再次凍存。
☆操作簡(jiǎn)便—周末免換液的培養(yǎng)日程, 使用隔天換液 (周一，周三，周五) 而不是每天換液 ☆無需準(zhǔn)備復(fù)雜的overlays或sandwich培養(yǎng)。
產(chǎn)品應(yīng)用
肝臟疾病建模Liver disease modeling
肝毒性評(píng)估Hepatotoxicity assessment
慢性中毒 Chronic toxicity
組份：250mL培養(yǎng)基
250 ml Cellartis Power Primary HEP Medium (Cat. No. Y20020)
自備試劑：肝細(xì)胞+膠原蛋白或膠原蛋白預(yù)包被培養(yǎng)板+PBS
•General cell culture equipment
•Human cryoplateable primary hepatocytes with the providers recommended thawing and plating medium
•Collagen I from rat tail (e.g., Corning Cat. No. 354236) or pre-coated Collagen I plates (e.g., Corning BioCoat™ Cat. No. 354407 or 354408)
•PBS Dulbecco's with Ca2+ & Mg2+ (D-PBS +/+)
If applicable:
• Penicillin-Streptomycin (PEST) for your thawing and plating medium (see section V.A.1)
培養(yǎng)性能
使用Cellartis Power Primary HEP Medium分別培養(yǎng)來源于三個(gè)不同品牌的、復(fù)蘇接種的人原代肝臟細(xì)胞。
Cryoplateable human primary hepatocytes from three different vendors (1, 2, and 3) cultured in Cellartis Power Primary HEP Medium. Cells were cultured as described and images were taken 1 (Panel A), 7 (Panel B), 14 (Panel C), 21 (Panel D) and 28 (Panel E) days after thawing. For all images, the scale bar is 100 μm
新型的Cellartis Power Primary HEP Medium是專門研發(fā)的、用于長(zhǎng)期維持培養(yǎng)的人原代肝臟細(xì)胞培養(yǎng)基。目前市場(chǎng)上銷售的原代肝臟細(xì)胞培養(yǎng)都配套有優(yōu)化的培養(yǎng)體系，主要有2D和3D培養(yǎng)兩種形式。2D貼壁培養(yǎng)，操作簡(jiǎn)便，但是培養(yǎng)的肝細(xì)胞在2-7天失去表型，而且要求每天換液。3D懸浮培養(yǎng)雖然可以延長(zhǎng)肝臟細(xì)胞功能特性維持的時(shí)間，但是在操作上比較復(fù)雜，需要制備overlay進(jìn)行sandwich 培養(yǎng) (Liu et al. 1999)或借助生物反應(yīng)器Bioreactors (Hoffman et al. 2012)等，成本也較高。Cellartis Power Primary HEP Medium可以支持方便操作的2D培養(yǎng)，多種品牌的原代肝臟細(xì)胞可以存活并維持功能特性4周，以及這款培養(yǎng)基更加人性化，支持隔天換液和周末免換液。
華雅再生醫(yī)學(xué)旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司主營(yíng)細(xì)胞治療和精準(zhǔn)醫(yī)療等產(chǎn)品，2018年與TAKARA繼續(xù)合作，授權(quán)代理（上海）其旗下Cellartis干細(xì)胞研究制品和Rubicon單細(xì)胞文庫構(gòu)建試劑。Cellartis公司位于瑞典，擁有iPS細(xì)胞等干細(xì)胞分化為肝細(xì)胞及臟器細(xì)胞的分化誘導(dǎo)技術(shù)和ES細(xì)胞，ips細(xì)胞，分化細(xì)胞等干細(xì)胞相關(guān)產(chǎn)品。
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